Two-dimensional gel electrophoresis and immunoblotting of Campylobacter outer membrane proteins
نویسندگان
چکیده
منابع مشابه
Two-dimensional gel electrophoresis and immunoblotting of Campylobacter outer membrane proteins.
We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flagellar band with a molecular mass of 63 kilodaltons (kDa) demonstrated previousl...
متن کاملTwo-dimensional gel electrophoresis and immunoblotting of Campylobacter pylori proteins.
Whole-cell, outer-membrane protein, flagellum-associated antigens and partially purified urease of Campylobacter pylori were analyzed by two-dimensional gel electrophoresis. C. pylori strains were readily distinguished from strains of Campylobacter jejuni, C. coli, and C. fetus by absence of major outer membrane proteins with Mrs of 41,000 to 45,000. C. pylori strains also lacked the acidic sur...
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Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction b...
متن کاملTwo-dimensional electrophoresis of membrane proteins.
One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies iso...
متن کاملimmunogens of brucella abortus s19 identified by two-dimensional gel electrophoresis and immunoblotting
background: lipopolysaccharides (lpss) and several antigenic proteins of brucella have been considered for preparation of diagnostic reagents and subunit vaccines. the objective of this study was to identify and compare immunogens of b. abortus s19 which induce humoral immune responses in human, goat and rabbit. material and methods: the bacterial whole cell extract was prepared in extraction...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1987
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.55.7.1564-1572.1987